Fig. 1: Experimental concept of hybrid PA/fast ULM imaging.
From: Hybrid photoacoustic and fast super-resolution ultrasound imaging
The dual in vivo images are generated and processed with the following protocols: a Animals are injected with microbubble solution before (or during) imaging. b During the DAQ, multi-wavelength photoacoustic (PA) imaging and plane-wave fast ultrasound imaging are recorded alternately at each position. The minimum DAQ time for fast ultrasound localization microscopy (ULM) imaging is determined by the characterization time of an averaged pixel saturation curve. c Ultrasound (US) images and PA images of multiple wavelengths are stored and processed separately with procedures shown in (d) and (e). d Sparsity-constrained (SC)-ULM imaging procedure includes three main steps: clutter filtering, SC recovery over frames, and location accumulation. e Blood oxygen saturation (sO2) PA images are generated through linear spectral unmixing from multi-wavelength PA images.
