Fig. 2: Genes and genetic modifications affecting ATS1.

a Top panel: Representative fluorescence microscopy images showing the effect of double deletion of the Hsp70 genes SSA1 and SSA2 on ATS1-GFP formation. Cells were grown at 22 °C. Bottom panel: Quantifications of the GFP intensity ratio of inclusion to cytoplasm. Quantifications of the fraction of cells with ATS1 inclusions. Data are presented as mean values +/− SEM. n = 3 independent experiments. Unpaired t-test (two-tailed): **p < 0.0054, ****p < 0.0001. b Domain architecture of Hsp104. Hsp104 is composed of an N-terminal domain (NTD), nucleotide-binding domain 1 (NBD1), middle domain (MD), nucleotide-binding domain 2 (NBD2), and C-terminal domain (CTD). NBD1 and NBD2 harbor the ATP binding sites ATP1 and ATP2, respectively. c Top panel: Representative fluorescence microscopy images showing the influence of the NBD and oligomerization of Hsp104 on inclusion formation of ATS1. Cells were grown at 30 °C. Bottom panel: Quantifications of the GFP intensity ratio of inclusion to cytoplasm. Quantifications of the fraction of cells with inclusions. Data are presented as mean values +/− SEM. n = 3 independent experiments. All scale bars within this figure represent 2 µm.