Fig. 4: Artificial sequestration of aggregates to non-canonical sites can be achieved by other ATS constructions.

a Representative fluorescence microscopy images showing the targeting of Hsp104 inclusions to the endosome by fusing Hsp104-GFP to Snf7 (ATS2). b Targeting of Hsp104 inclusions to eisosomes by fusing Hsp104-GFP to Pil1 (ATS3). c Left panel: Representative fluorescence microscopy images of the artificial targeting of mHtt103QP-mCherry aggregates to endosomes by using Hsp104-GFP-Snf7. Right panel: Quantifications of mHtt targeting efficiency. The expression of mHtt103QP-mCherry was induced by galactose for 4.5 h. Data are presented as mean values +/− SEM. n = 3 independent experiments. Unpaired t-test (two-tailed): ***p = 0.0001. d Left panel: Representative fluorescence microscopy images of the artificial targeting of mHtt103QP-mCherry aggregates to eisosomes by using Hsp104-GFP-Pil1. Right panel: Quantifications of mHtt targeting efficiency. The expression of mHtt103QP-mCherry was induced by galactose for 4.5 h. Data are presented as mean values +/− SEM. n = 3 independent experiments. Unpaired t-test (two-tailed): ****p < 0.0001. e Growth curves (left panel) and doubling time (right panel) of cells with artificial targeting of mHtt103QP-mCherry aggregates to the eisosomes by using Hsp104-Pil1. mHtt103QP was under the control of a constitutive GPD promoter. Data are presented as mean values +/− SEM. Control: n = 3; Hsp104-Pil1: n = 6 independent experiments. f Growth curves (left panel) and doubling time (right panel) of cells with artificial targeting of mHtt103QP-mCherry aggregates to the eisosomes by using Hsp104-Pil1. mHtt103QP was under the control of an inducible GAL1-promoter. Data are presented as mean values +/− SEM. n = 3 independent experiments. All scale bars within this figure represent 2 µm.