Fig. 5: Screening for ‘inclusion generators’ other than Hsp104.
From: Artificial Hsp104-mediated systems for re-localizing protein aggregates
a Scheme for screen strategy. GFP binding protein (GBP) was fused to Pea2 and crossed with the GFP library using SGA technology. Cells were screened for a phenotype with a strong artificial inclusion of a chimera consisting of the GFP-labeled gene of interest and Pea2. b Functional clustering of hits for inclusion generators. Edges (lines) represent published physical protein–protein interactions and functional associations. Nodes represent the screen hits. c Screen data output for product of inclusion-to-cytoplasm-ratio and fraction of cells with inclusions (ratio × fraction). Data are presented as mean values +/− SEM. n = 3 independent experiments. d Gene ontology biological process analysis of screen hits.
