Fig. 6: Cellular effects of exporting protein aggregates from mother cells.
From: Artificial Hsp104-mediated systems for re-localizing protein aggregates
a Scheme of labeling mother cells with sulfo-cyanine5-NHS ester. Daughters stay unlabeled and can be distinguished from mother cells using flow cytometry. Subsequently, a cell death analysis was performed. Created with BioRender.com. b Quantification of the decrease of mothers having mHtt103Q inclusions. Data are presented as mean values +/− SEM. n = 3 independent experiments. c Cell death analysis by flow cytometry of Cyanine5-labeled mother cells after induction of mHtt103Q expression, depicted as decrease of dead labeled ATS1 mother cells in relation to non-ATS1 mother cells. Data are presented as mean values +/− SEM. Without mHtt: n = 3; with mHtt: n = 4 independent experiments. Unpaired t-test (two-tailed): *p = 0.0314. d Replicative lifespan analysis via micromanipulation of cells with inducible ATS1. Median lifespan is indicated in parentheses.
