Fig. 2: Poststress ventral hippocampal SWRs differ between stress-susceptible and stress-resilient mice.
From: Hippocampal sharp wave ripples underlie stress susceptibility in male mice
a (Left) LFP recordings were obtained from the vHC after injection of AAV-shCalb1. (Right) Histological confirmation of a recording site in the vHC CA1 cell layer in a cresyl-stained section. The arrowhead indicates the track of the electrode. The same results were obtained from 72 mice with electrodes implanted in the vHC. b Unfiltered and bandpass-filtered (150–250 Hz) LFP traces of a representative ventral hippocampal SWR. c Schematic illustration of the recording time course. The difference in poststress neuronal activity between the prestress and 0–2 h poststress period was computed on day 1, and several mice were subjected to an SI test on day 2. d Changes in the rates of ventral hippocampal SWRs in SD mice (black, n = 23 mice). For comparison, the same recordings were performed for non-SD mice (gray, n = 6 mice). 0–30-min pre versus 0–30-min post periods, Z = 2.65, *P = 0.024, two-sided Mann–Whitney U test followed by Bonferroni correction. The data are the mean ± SEM. e The data from the SD mice shown in d were plotted separately for mice that showed stress susceptibility (magenta, n = 12 mice) and resilience (blue, n = 5 mice), as defined by a subsequent SI test. 0–30-min pre versus 0–30-min post periods, Z = 3.02, *P = 0.0075; 0–30-min pre versus 30–120-min post periods, Z = 3.15, *P = 0.0049, two-sided Mann–Whitney U test followed by Bonferroni correction. The data are the mean ± SEM. f Relationship between the poststress ΔSWR rate and SI ratio. Each dot represents a mouse (n = 17 mice). R = –0.64, P = 0.0057. g Same as d but for mice injected with AAV-shCalb1 into the vHC (n = 8 mice). P > 0.05 in all post periods versus the 0–30-min prestress period, two-sided Mann–Whitney U test followed by Bonferroni correction. The data are the mean ± SEM. h Same as f but for mice injected with AAV-shCalb1. No significant Pearson’s correlation (P = 0.63, two-sided) was found between the poststress ΔSWR rate and SI ratio.
