Fig. 10: Induction of robust and long-lived germinal center responses by BG505 UFO trimer-presenting SApNPs. | Nature Communications

Fig. 10: Induction of robust and long-lived germinal center responses by BG505 UFO trimer-presenting SApNPs.

From: Single-component multilayered self-assembling protein nanoparticles presenting glycan-trimmed uncleaved prefusion optimized envelope trimers as HIV-1 vaccine candidates

Fig. 10

a Top: Representative immunohistological images of BG505 UFO-10GS-I3-01v9 SApNP vaccine-induced germinal centers (GCs) at 2 weeks after a single-dose injection (10 μg/injection, 40 μg/mouse). Bottom left: GC B cells (GL7+, red) adjacent to FDCs (CD21+, green) in lymph node follicles. Bottom Right: Tfh cells in the light zone (LZ) of GCs. Scale bars = 500 and 100 μm for complete lymph node and enlarged image of a follicle, respectively. b and c Quantification of GCs using immunofluorescent images at 2, 5, and 8 weeks after a single-dose injection or at 2 and 5 weeks after the boost, which occurred at 3 weeks after the first dose (n = 5–10 mice/group). Data were collected from 35–108 lymph node follicles. The GC/FDC ratio and GC size were determined and plotted here. d and e Representative immunohistological images of GCs induced by the BG505 UFO trimer, E2p, I3-01v9, and GT I3-01v9 SApNP vaccines at 8 weeks using a single-dose or prime-boost regimen. Scale bar = 50 μm for each image. f and g Quantification of GCs by flow cytometry after a single-dose or prime-boost injections (n = 5 mice/group). Percentage and number of GC B cells and Tfh cells were determined and plotted. The data points are shown as mean ± SD. The data were analyzed using one-way ANOVA followed by Tukey’s multiple comparison post hoc test for each time point. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

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