Fig. 4: Antigenicity of BG505 UFO Env immunogens with wildtype and modified glycans. | Nature Communications

Fig. 4: Antigenicity of BG505 UFO Env immunogens with wildtype and modified glycans.

From: Single-component multilayered self-assembling protein nanoparticles presenting glycan-trimmed uncleaved prefusion optimized envelope trimers as HIV-1 vaccine candidates

Fig. 4

a Antigenic profiles of BG505 UFO trimer (top) and its three SApNPs based on FR, E2p-L4D, and I3-01v9-L7P (bottom) with different glycan treatments (wildtype, expressed in the presence of kifunensine, and endo H treatment). A total of 14 NAbs/bNAbs and 9 non-NAbs were tested by BLI. Sensorgrams were obtained from an Octet RED96 using an antigen titration series of six concentrations (starting at 266.7 nM for the UFO trimer, 14.9 nM for the FR SApNP, 5.5 nM for the E2p and I3-01v9 SApNPs followed by two-fold dilutions) and are shown in Fig. S4. The peak values at the highest concentration are summarized in the matrix, in which cells are colored in red and green for (b)NAbs and non-NAbs, respectively. Higher color intensity indicates greater binding signal measured by Octet. b Negative-stain EM analysis of the glycan-trimmed BG505 UFO trimer in complex with bNAb PGT128. Examples of 2D class images (top) and comparison with a cryo-EM model of BG505 SOSIP/PGT128 complex (bottom, EMDB-3121, cornflower blue). c Negative-stain EM analysis of the glycan-trimmed BG505 UFO trimer in complex with bNAb VRC01. Examples of 2D class images (top) and superposition of the 3D reconstruction onto two previously reported BG505 SOSIP/VRC01 models derived from negative-stain EM (EMDB-6252, orange) and cryo-EM (PDB ID: 6V8X, cyan and green).

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