Fig. 5: Immunogenicity of HIV-1 UFO Env immunogens with wildtype glycans in mice and rabbits. | Nature Communications

Fig. 5: Immunogenicity of HIV-1 UFO Env immunogens with wildtype glycans in mice and rabbits.

From: Single-component multilayered self-assembling protein nanoparticles presenting glycan-trimmed uncleaved prefusion optimized envelope trimers as HIV-1 vaccine candidates

Fig. 5

a Schematic representation of the mouse immunization regimen for two BG505 UFO trimer-presenting E2p and I3-01v9 SApNPs. b Neutralization of tier 2 clade A BG505.T332N and tier 1 clade B SF162 by purified mouse IgG from week 11 (n = 8 mice/group). c Percent neutralization of week 11 mouse IgG from four vaccine responders against BG505.T332N and its I396R mutant. Vaccine responders are those subjects with IC50 < 300 μg/ml in IgG neutralization against BG505.T332N. Color coding indicates the level of reduction in % neutralization relative to BG505.T332N. In both b and c, the TZM-bl assay was performed in duplicate, starting at an IgG concentration of 300 μg/ml (unless otherwise indicated by an asterisk) followed by three-fold dilutions. Error bars in b represent the difference between these duplicate values at each concentration tested for each sample. d Schematic representation of the rabbit immunization protocol. Seven groups of rabbits, 6 per group, were immunized to test the BG505 UFO trimer and its FR, E2p, and I3-01v9 SApNPs, with a cocktail group added for each SApNP by mixing equal amounts of three SApNPs that display BG505 UFO, CH505 UFO-BG, or H078.14 UFO-BG trimers (termed Env mix). e Longitudinal analysis of rabbit plasma neutralization between weeks 2 and 28 (n = 6 rabbits/group). All samples were tested in duplicate with a 100-times starting dilution. Average ID50 values for each group against BG505.T332N are shown with the SEM. Groups that were immunized with the same vaccine platform are plotted on the same graph: trimer (red), FR (dark/light purple), E2p (dark/light green) and I3-01v9 (dark/light blue). Week 0 data are omitted due to nonspecific background signals. f Percentage neutralization of select week 26 rabbit plasma (≥ 35% neutralization against BG505.T332N at the first dilution) against BG505.T332N and four glycan hole mutants. For each mutant virus, rabbit plasma was tested in duplicate, starting at 100-times dilution followed by three-fold dilutions. Results are shown for BG505.T332N from e and the mutant viruses at the 100-times dilution. Color coding indicates the level of reduction in % neutralization relative to BG505.T332N. Mouse and rabbit images created with BioRender.com.

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