Fig. 9: Interaction of BG505 UFO trimer-presenting SApNPs with FDCs and phagocytic cells in lymph nodes. | Nature Communications

Fig. 9: Interaction of BG505 UFO trimer-presenting SApNPs with FDCs and phagocytic cells in lymph nodes.

From: Single-component multilayered self-assembling protein nanoparticles presenting glycan-trimmed uncleaved prefusion optimized envelope trimers as HIV-1 vaccine candidates

Fig. 9

a TEM images of E2p SApNPs (yellow arrows) and AddaVax adjuvant (green arrows) presented on the FDC dendrites in a lymph node at 12 h after a single-dose injection (2 footpads, 50 μg/footpad). b and c TEM images of I3-01v9 SApNPs (yellow arrows), wildtype and glycan-trimmed (GT), aligned on FDC dendrites without observable aluminum phosphate (AP) adjuvant at 12 h after a single-dose injection. d TEM images of E2p SApNPs (yellow arrows) and AddaVax adjuvant (green arrows) colocalized on the surface or inside endolysosomes of a macrophage at 12 h after injection. e and f TEM images of I3-01v9 SApNPs (yellow arrows), wildtype and glycan-trimmed (GT), and aluminum phosphate adjuvant (green arrows) internalized inside endolysosomes of a macrophage, with only SApNPs (yellow arrows) on the macrophage surface at 48 h for e, or 12 h for f, after the injection. TEM images were performed on 2 popliteal lymph nodes for each SApNP construct.

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