Table 1 Binding affinities for host-guest interactions between the macrocycle P[5]a and HSLs, determined through dye displacement with the MO indicator

From: Repurposing host-guest chemistry to sequester virulence and eradicate biofilms in multidrug resistant Pseudomonas aeruginosa and Acinetobacter baumannii

HSL

kD (M) in 0 mM NaCl

kD (M) in 50 mM NaCl

k−1 MO-P[5]a

(0.68 ± 0.08) nM

(9.82 ± 0.06) µM

k−2 3-OH-C14:1 (Cin)

(2.00 ± 0.20) µM a

(2.58 ± 0.07) µM (a)

k−2 3-oxo-C12 (Las)

(1.69 ± 0.05) µM

(5.90 ± 0.02) µM

k−2 3-oxo-C8 (Tra)

(18.60 ± 0.60) µM

(0.12 1 ± 0.02) mM

k−2 3-oxo-C6 (Lux)

(0.83 ± 0.04) mM

(1.36 ± 0.03) mM

k−2 pC (Rpa)

(0.65 ± 0.05) mM

(4.36 ± 0.09) mM

k−2 C4 (Rhl)

(15.00 ± 0.60) mM

– b

  1. a3OH-C14:1 dataset is limited due to strong scattering. bSignal increase is insignificant to be fitted
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