Fig. 1: Asymmetric reconstructions of capsids and in situ portals of the human cytomegalovirus (HCMV) A- and B-capsids.

Composite structures of the HCMV B-capsid (a) and A-capsid (b), respectively. The front-halves of both capsid shells (light blue) are removed to show their internal components. The front-right quarter of the scaffold in (a) is removed to show the inner three-layer scaffold (the inner core and middle band are radially colored from dark to medium purple and the outer shell is highlighted in red). The portal vertices are colored as indicated below in (c). c Close-up views of the portal vertex regions in B- (left), A- (middle) and virion (right) capsids. The capsid shell components of the portal vertex are colored by protein. The portal is colored by domain. For both B- (left) and A-capsid (middle), the composite cryoEM maps were assembled from reconstructions of C1 capsid (light blue), C5 portal vertex (CVSC, salmon; Ta, white; Tc, cyan; portal turret, magenta; portal 10-helix anchor, violet) and C12 portal main body (yellow). The portal-bound scaffold fragments from C12 reconstruction of portal in B-capsid are highlighted in red. For virion capsid (right), the composite cryoEM map were assembled from reconstructions of C1 capsid (light blue, EMD-31292), C1 portal vertex (pDNA, orange. EMD-31290), C5 portal vertex (CVSC, salmon; Ta, white; Tc, cyan; portal 10-helix anchor, violet. EMD-31297), C6 portal (portal turret: magenta. EMD-31299) and C12 portal main body (yellow. EMD-31295). d Top-views of in situ portals of B- (left), A- (middle), and virion (right) capsids. Portal turrets are highlighted to show the symmetry difference in arrangement between DNA-devoid and DNA-filled capsids.