Fig. 4: Dysregulated HSC pool is rescued by re-establishing BM LCM.

a Homing of MK-LCM^WT/WT and MK-LCM^Δ/Δ HSC into the BM of C57Bl/6 mice 15 h post-transplant (n = 3, biological replicates, from one experiment). b Limiting dilution transplant outcome for mice receiving varying numbers of MK-LCM^WT/WT or MK-LCM^Δ/Δ HSC (n = 5 for MK-LCM^WT/WT cell doses of 100 and 300, n = 10 for MK-LCM^WT/WT cell doses of 10 and 30, n = 5 for MK-LCM^Δ/Δ cell doses of 10, 30, 1k, 3k and 10k and n = 10 for MK- LCM^Δ/Δ cell doses of 100 and 300, all biological replicates, from two experiments). c Schematic for the analysis of hematopoietic potential of GFP⁺MK-LCM^WT/WT plus RFP⁺MK-LCM^Δ/Δ or RFP⁺MK-LCM^Δ/Δ only HSC. d Percentage of donor contribution for a competitive transplant using equal numbers of MK-LCM^WT/WT plus MK-LCM^Δ/Δ HSC (blue), or MK-LCM^Δ/Δ HSC only (green). e Frequency of hematopoietic stem and progenitors and f HSC in mice reconstituted with MK-LCM^WT/WT plus MK-LCM^Δ/Δ or MK-LCM^Δ/Δ only HSC. g The number of HSC in the PB and spleen (h) following reconstitution with MK-LCM^WT/WT plus MK-LCM^Δ/Δ or MK-LCM^Δ/Δ only HSC. i Total MK number, j number of LCM and k number of MK of MK-LCM^Δ/Δ origin following reconstitution with MK-LCM^WT/WT plus MK-LCM^Δ/Δ or MK-LCM^Δ/Δ only HSC. c–k n = 4, biological replicates, from one experiment. l Schematic for competitive limiting dilution transplant (n = 5 per group, biological replicates). m Frequency of HSC in the BM relative to total BM MK-LCM^Δ/Δ reconstitution (n = 43 mice from 4 transplants, dotted line represents the frequency of HSC in MK-LCM^WT/WT BM). n Correlation between the frequency of HSC in BM as compared to the proportion of LCM in the BM of reconstituted mice (two-phase exponential decay, dotted line = 0.001%, n = 41 mice from 4 transplants). Statistical analysis used unpaired two-sided Student’s t-test, p values indicated for (a, d–f, h–k), two-sided Mann–Whitney test (g), Poisson analysis (b), linear regression statistics (m), two-phase exponential decay (n). Source data are provided as a Source Data File. Error bars = SEM.