Fig. 3: Cas9-mediated cleavage of incoming plasmids can bias their transfer to targeted recipients.
From: Addressable and adaptable intercellular communication via DNA messaging
a Schematic of the experimental setup. Senders (S) and Receivers (R) carrying one of two plasmid variants are grown together in a coculture, and selective plating is used to isolate them, as well as the transconjugants (T), from the mixed culture. Note that transconjugants will appear on the receiver-selecting plates, so R is the total density of receivers in the population (Methods). b Endpoint strain densities, measured in colony forming units (CFUs) per mL of culture. (c) Transfer rates, calculated as T/(S ∗ R), of the message plasmid in each of the conditions in (b). Dots show the values from three biological replicates measured on different days, and bars depict the geometric mean of these values. Km kanamycin, Cm chloramphenicol, Ap apramycin, Sp spectinomycin, Cin = OHC14-HSL. Source data are provided as a Source Data file.
