Fig. 4: Kinetic resolution of photoprotective gene and protein expression at different light and CO₂ availabilities.

Cells were acclimated overnight at LL (15 µmol photons m⁻² s⁻¹) bubbled with air (labelled “air”). At t = 0 the light intensity was raised to 600 µmol photons m⁻² s⁻¹ under air bubbling or bubbling with 5% CO₂ and mRNA and protein were followed for 25 h. a LHCSR1, LHCSR3 and PSBS mRNA accumulation. (n = 3 biological samples, mean ± s.d.). The p-values for the comparisons of CO₂ conditions to air for t = 1, 4, 8, 24 and 25 h are based on two-way ANOVA Šídák’s multiple comparisons test of log10 transformed mRNA data as indicated in the graphs (*P < 0.005, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns, not significant). Exact p-values can be found at the Source Data file. b Immunoblot analyses of LHCSR1, LHCSR3 and ATPB (loading control). Representative dataset of experiment repeated three times.