Fig. 3: EhaF inhibits IRF3 phosphorylation during EHEC infection. | Nature Communications

Fig. 3: EhaF inhibits IRF3 phosphorylation during EHEC infection.

From: A bacterial autotransporter impairs innate immune responses by targeting the transcription factor TFE3

Fig. 3

a–f, h, i Immunoblots for the indicated proteins in the lysates of BMDMs infected with wild-type EHEC or ΔEhaF for the indicated time. g, j Immunoblots for the indicated proteins in the nuclear and cytoplasmic fractions of BMDMs infected with wild-type EHEC or ΔEhaF for the indicated time. a–i, Combined data (mean ± SEM) from the densitometric analysis of indicated proteins relative to indicated control proteins from three independent experiments is provided next to each blot. *g, j images are from the same immunoblot membrane probed for p65, pIRF3, PARP and tubulin, hence same PARP and tubulin images in both. Statistical significance was assessed using two-way ANOVA followed by Tukey’s post-test. p < 0.05 indicated statistical significance. ns=not significant. Multiplicity adjusted p values are presented. Source data are provided as a Source Data file.

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