Fig. 7: EhaF-mediated innate immune suppression promotes EHEC pathogenesis. | Nature Communications

Fig. 7: EhaF-mediated innate immune suppression promotes EHEC pathogenesis.

From: A bacterial autotransporter impairs innate immune responses by targeting the transcription factor TFE3

Fig. 7

a Intracellular bacterial load in BMDMs treated with 10 ng/ml of IFNβ 30 min prior to infection with EHEC or ΔEhaF for the indicated times. b Bacterial adherence in Caco2 cells treated with 10 ng/ml of human IFNβ 60 min prior to infection with EHEC or ΔEhaF for the indicated times. c–e Bacterial colonization at the indicated days post-infection determined by viable fecal counts (c, d) and survival (e) of streptomycin-treated mice intraperitoneally (i. p.) injected with 250 µg of an isotype control antibody or anti-IFNAR antibody one day prior to and on day 1 and 3 after oral gavaging with 1 × 1010 of EHEC [n = 7 for isotype and anti-IFNAR (c, d) and n = 10 for isotype and anti-IFNAR (e)]. f–i Bacterial colonization on days 2 and 3 of infection determined by serial dilution and plating of feces (f, g) or of colon homogenates (h, i) of streptomycin-treated mice orally gavaged with 1 × 1010 of EHEC or ΔEhaF (f, g, n = 7 for EHEC and ΔEhaF each for day 2 and n = 8 for EHEC and ΔEhaF each for day 3; h, i n = 7 for EHEC and n = 6 for ΔEhaF for day 2 and n = 6 for EHEC and n = 5 for ΔEhaF for day 3). j, k Levels of IFNβ or IL-6 in the colon homogenates of streptomycin-treated mice orally gavaged with 1 × 1010 of EHEC or ΔEhaF on day 2 post-infection [n = 4 for UI, n = 10 for EHEC and ΔEhaF (j) and n = 4 for UI, n = 7 for EHEC and ΔEhaF (k)]. l Survival of streptomycin-treated mice orally gavaged with 1 × 1010 of EHEC (n = 9) or ΔEhaF (n = 9). a, b Data (mean ± SEM) were from three independent experiments and each dot is a mean of each experiment’s technical replicates. Statistical significance was assessed using two-way ANOVA followed by Tukey’s post-test. c–l Combined data from two independent experiments are shown. Statistical significance was assessed using two-sided unpaired t-test (c, d, f–i) or one-way ANOVA followed by Tukey’s post-test (j, k) or Mantel-Cox test (e, l). p < 0.05 indicated statistical significance. Source data are provided as a Source Data file.

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