Fig. 8: p140Cap controls the stability of the destruction complex. | Nature Communications

Fig. 8: p140Cap controls the stability of the destruction complex.

From: p140Cap inhibits β-Catenin in the breast cancer stem cell compartment instructing a protective anti-tumor immune response

Fig. 8: p140Cap controls the stability of the destruction complex.The alternative text for this image may have been generated using AI.

a Confocal images showing the number of Axin1-GFP punctae in Mock and p140Cap-TuBo cells. The nuclei were counterstained by DAPI. Scale bar 10 μm. Data are representative of n = 5 experimental repeats and shown as mean ± SEM; two-tailed unpaired t test. b Confocal images showing the colocalization of Axin1-GFP and p140Cap-RFP in punctae in Mock-TuBo cells co-transfected with Axin1-GFP and p140Cap-RFP. Scale bar 10 μm. Data are representative of n = 5 experimental repeats and shown as mean ± SEM; two-tailed unpaired t test. c Selected frames of FRAP experiments before and after bleaching, and throughout the recovery of Axin1-GFP in TuBo cells transfected with Axin1-GFP alone or in combination with p140Cap-RFP. Immobile fraction and half-life of GFP-Axin1. Scale bar 1 μm and 10 μm. Cell number > 31, data are representative of n = 3 experimental repeats and shown as mean ± SEM; two-tailed unpaired t test; 2way ANOVA for FRAP.

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