Fig. 3: PAM preferences of enOsCas12f1 and enRhCas12f1. | Nature Communications

Fig. 3: PAM preferences of enOsCas12f1 and enRhCas12f1.

From: Engineered CRISPR-OsCas12f1 and RhCas12f1 with robust activities and expanded target range for genome editing

Fig. 3

a PAM preferences of OsCas12f1, enOsCas12f1 and Un1Cas12f1_ge4.1 analyzed by GFP activation system. Target sequence: CCATTACAGTAGGAGCATAC. Values and error bars represent mean and s.d. (n = 3). b Comparison of RhCas12f1- and enRhCas12f1-preffered PAM. Target sequence: CCATTACAGTAGGAGCATAC. Values and error bars represent mean and s.d. (n = 3). c, d Validation the PAM preferences of enOsCas12f, enRhCas12f1 and Un1Cas12f1_ge4.1 at endogenous loci. Values and error bars represent mean and s.d. (n = 3). e, f Summary of indel efficiencies of enOsCas12f1, Un1Cas12f1_ge4.1, and enRhCas12f1. Values and error bars represent mean and s.d. from biologically independent experiments. Source data are provided as a Source Data file.

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