Fig. 4: Comprehensive validation of genomic editing efficiency of enOsCas12f1 and enRhCas12f1 in human cells. | Nature Communications

Fig. 4: Comprehensive validation of genomic editing efficiency of enOsCas12f1 and enRhCas12f1 in human cells.

From: Engineered CRISPR-OsCas12f1 and RhCas12f1 with robust activities and expanded target range for genome editing

Fig. 4

a Distribution of all exon-located target sites that are accessible for enOsCas12f1 (5’-NTTC PAM), enRhCas12f1 (5’-CCCA PAM), and Un1Cas12f1_ge4.1 (5’-TTTR PAM), and the indel frequencies are indicated by mean values of three replicates, determined by NGS. The exon (gray solid squares) is connected by intron (lines), and UTRs are shown as hollow boxes. b Indel frequencies of enOsCas12f1, enRhCas12f1 and Un1Cas12f1_ge4.1 at endogenous genomic loci. Each dot represents single target site, value means average of three replicates. Bars represent means. c Comparison of editing efficiencies of enOsCas12f1 and Un1Cas12f1_ge4.1 targeted by same sgRNAs at PCSK9 and TTR loci. Values and error bars represent mean and s.d. (n = 3). d Average indel frequency of enOsCas12f1 and Un1Cas12f1_ge4.1 at 5’-TTC PAM and 5’-TTTR PAM target sites. Each dot represents single target site, value means average of 3 replicates. Error bars represent mean and s.d. e Comparison of editing efficiencies of enOsCas12f1 and SpG targeted by same sgRNAs at endogenous target loci. Values and error bars represent mean and s.d. (n = 3). f The distribution of mutant alleles by enOsCas12f1-mediated disruption at TTR locus, the top 10 mutant alleles are represented. Source data are provided as a Source Data file.

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