Fig. 1: Bacteroidetes growth on mycobacterial arabinogalactan.
From: Identification of d-arabinan-degrading enzymes in mycobacteria
A Schematic of the structure of mycobacterial mycolyl-arabinogalactan-peptidoglycan complex. Succinate (‡) and galactosamine (*) modifications are non-stoichiometric. B Growth of selected Bacteroidetes species on arabinogalactan as monitored by change in OD600nm. C Ion chromatography with pulsed amperometric detection (IC-PAD) analysis of culture supernatants for bacteria grown on mycobacterial AG as a sole-carbon source. Production of arabinose (green star) and galactose (yellow circle) are indicated and compared to standards. Data from D. gadei is also shown as an inset. D Schematics of PUL47 and 39 from B. finegoldii, whose homologs in B. cellulosilyticus were identified by RNAseq as being upregulated during growth on AG. E TLC analysis of endpoint reaction products of B. finegoldii enzymes with C. glutamicum ∆ubiA galactan. Filled circles indicate the presence of a given reaction component. The major product appears to be galactobiose suggesting endo-activity. A detailed analysis of this enzyme is beyond the scope of this study. Source data are provided in the Source Data file.
