Fig. 3: Distinct activity and response properties of one-channel and multi-channel clusters.
From: Associations between in vitro, in vivo and in silico cell classes in mouse primary visual cortex
a One-channel FS and RS clusters show distinct interspike interval (ISI) distributions (Mann–Whitney U test, two-sided, p = 0.0, total 2,900,284 spikes of FS, 4,586,637 spikes of RS). Response properties of the multi-channel clusters to drifting gratings show that RS1–3 exhibit distinct properties in the overall excitability (spike rate, coefficient of variation: CV, local variation: LV, n = 419 RS1, n = 173 RS2, n = 153 RS3) and stimulus-dependent response characteristics (f1/f0, modulation index, lifetime sparseness, n = 430 RS1, n = 182 RS2, n = 156 RS3). Kruskal–Wallis H-test; p-values corrected using the Holm–Bonferroni method for multiple tests. *p < 0.05, **p < 0.01, ***p < 0.001. b Phase distribution of examples of an FS (blue) and RS (red) unit at theta, alpha, beta, low gamma (lgamma), high gamma (hgamma) frequency band (black arrow: preferred phase and kappa). c Left: The percentage of phase-locked units of one-channel FS (n = 203) and RS (n = 745) clusters at different LFP frequency bands: theta, alpha, beta, low gamma, and high gamma. Two sample z tests for proportions with p values were corrected by the Holm–Bonferroni method for multiple tests. Right: kappa and preferred phase. Data are presented as mean ± SEM (standard error of the mean). Mann–Whitney U test, two-sided, *p < 0.05, **p < 0.01, ***p < 0.001. d, e Phase-locking analysis of multi-channel RS (d n = 419 RS1, n = 173 RS2, n = 153 RS3) and FS (e n = 100 FS1, n = 54 FS2, n = 49 FS3) clusters to ongoing oscillations in different LFP bands. Kruskal–Wallis H-test; p-values corrected using the Holm–Bonferroni method for multiple tests. *p < 0.05, **p < 0.01, ***p < 0.001. Source data are provided as a Source Data file.
