Fig. 5: Distinct cellular properties of multi-channel regular-spiking (RS1–3) clusters.
From: Associations between in vitro, in vivo and in silico cell classes in mouse primary visual cortex
a Clustering of spiny (SP) models using K-means clustering based on multi-channel extracellular action potential (EAP) features. Both the elbow method and density function analysis independently identify three multi-channel SP clusters (left: within-cluster sum of squares (WCSS) and density function, broken red line: the optimal number of clusters; right: model-based SP clusters; inset: mean EAP-waveform of each RS-population). SP1–3 and RS1–3 are shown using the multi-channel features 1/Vbelow and 1/Vabove (the inverse of spike propagation velocity below/above soma location). b Spike propagation along the simulated probe as a function of distance from the soma (channel with largest EAP amplitude) for the three SP classes, SP1–3 (gray lines: propagation of individual models; n = 5 SP1, n = 7 SP2, n = 3 SP3; colored lines: mean ± SD (standard deviation)). c The model-based classifier (random forest) trained on the multi-channel features (1/Vbelow and 1/Vabove) identifies SP1–3 (left: confusion matrix; middle: feature importance based on classifier; right: Sankey diagrams show the prediction on the test dataset). d Same layout as in (c), the experiment-based classifier was trained on multi-channel in vivo EAP features to discriminate between RS1–3. e Comparison between model conductances ascribed to SP1–3. The largest effect size across the conductances is found for axonal Ca_LVA. # indicates Cohen’s d effect size > 0.8. f Bifurcation distance (w) of one bifurcation node in the reconstructed morphology of a neuron is defined as the projection of the vector (v) from soma (S, red dot) to the position of the bifurcation node (N, blue dot) projected to a line (u) connecting the soma (S) to a node (L) in y-axis. g Morphology bifurcation distance above soma (left) and below soma (middle). Right: inverse of wave propagation velocity vs. the bifurcation distance (line: linear fit). h, i Intrinsic properties from in vitro experiments based on SP1–3 (subthreshold and spiking responses). j Comparison of cellular time constant (τ) and max spike rate (response to dc current injections) among in vitro experiments based on SP1–3. # indicates Cohen’s d effect size > 0.8. Source data are provided as a Source Data file.
