Fig. 2: Screening neutrophil-specific chimeric antigen receptor (CAR) structures with enhanced neutrophil-mediated anti-tumor activities. | Nature Communications

Fig. 2: Screening neutrophil-specific chimeric antigen receptor (CAR) structures with enhanced neutrophil-mediated anti-tumor activities.

From: CAR-neutrophil mediated delivery of tumor-microenvironment responsive nanodrugs for glioblastoma chemo-immunotherapy

Fig. 2: Screening neutrophil-specific chimeric antigen receptor (CAR) structures with enhanced neutrophil-mediated anti-tumor activities.The alternative text for this image may have been generated using AI.

a Schematic of various CAR structures. b Schematic of CAR #1 construct and targeted knock-in strategy at the AAVS1 safe harbor locus of human pluripotent stem cells (hPSCs). Vertical arrow indicates the AAVS1 targeting sgRNA. Red and blue horizontal arrows indicate primers for assaying targeting efficiency and homozygosity, respectively. HDR: homologous recombination repair. c Schematic of optimized neutrophil differentiation from hPSCs under chemically-defined conditions. d Cytotoxicity assays against U87MG glioblastoma cells were performed at different ratios of neutrophil-to-tumor target using indicated neutrophils. Data are represented as mean ± SD of five independent biological replicates, two-tailed Student’s t test. Reactive oxygen species (ROS) generation (e) and ELISA analysis of TNFα release (f) from different neutrophils after coculturing with U87MG cells were determined. n = 5 biologically independent samples. The data are represented as mean ± SD, two-tailed Student’s t test. Source data are provided as a Source Data file.

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