Table 4 Cox proportional hazards regression model of overall survival in patients with non-small cell lung cancer compared high and low densities, close with long distances and mixed with unmixed pattern relative to malignant cells, adjusted for clinicopathologic variables

From: Immune cellular patterns of distribution affect outcomes of patients with non-small cell lung cancer

Variable

B

SE

Wald

HR

95% CI for Exp(B)

P*

Histologic type (ADC vs SCC)

−1.103

1.081

1.042

0.332

0.040–2.759

0.307

Smoker (no vs yes)

1.702

1.938

.772

5.485

0.123–244.607

0.380

Tumor size (≤1.5 cm vs >3.8 cm)

−2.328

0.854

7.432

0.098

0.018–0.520

0.006

KRAS (wild-type vs mutant)

1.452

0.981

2.191

4.273

0.625–29.231

0.139

EGFR (wild-type vs mutant)

−1.411

1.207

1.367

.244

0.023–2.597

0.242

Low vs high densities

  CD3 + 

3.377

0.996

11.506

29.289

4.161–206.159

0.001

  CD3 + CD8 + 

2.882

1.126

6.545

17.847

1.962–162.338

0.011

  CD3 + CD8 + GZB + 

0.524

1.296

0.164

1.689

0.133–21.429

0.686

  CD3 + CD45RO + 

−2.725

1.669

2.665

0.066

0.002–1.727

0.103

  CD3 + CD8 + CD45RO + 

0.644

1.288

0.250

1.905

0.153–23.766

0.617

  CD3 + CD8negFOXP3 + 

0.913

0.670

1.854

2.491

0.670–9.268

0.173

  CD3 + CD45RO + FOXP3 + 

−4.203

1.485

8.008

0.015

0.001–0.275

0.005

  CD3 + PD-1+

−0.142

0.798

0.032

0.868

0.182–4.143

0.859

  CD3 + CD8 + PD-1+

4.311

1.405

9.414

74.550

4.746–1170.936

0.002

  CD3 + PD-L1 + 

8.036

1.729

21.612

3090.038

104.373–91482.972

0.000

  CD3 + CD8 + PD-L1 + 

0.486

1.224

0.157

1.625

0.148–17.880

0.692

  CD3 + PD-1+PD-L1 + 

−3.646

1.074

11.519

0.026

0.003–0.214

0.001

Mixed vs unmixed pattern

  CD3 + 

−3.073

1.124

7.471

0.046

0.005–0.419

0.006

  CD3 + CD8 + 

0.995

1.241

.643

2.704

0.238–30.771

0.423

  CD3 + CD8 + GZB + 

−0.593

.800

.551

.552

0.115–2.648

0.458

  CD3 + CD45RO + 

3.454

1.152

8.990

31.623

3.307–302.378

0.003

  CD3 + CD8 + CD45RO + 

0.526

1.196

0.193

1.692

0.162–17.628

0.660

  CD3 + CD8negFOXP3 + 

7.917

61.229

0.017

2743.024

0.000–3.601E + 55

0.897

  CD3 + CD45RO + FOXP3 + 

−1.087

0.844

1.661

0.337

0.065–1.761

0.197

  CD3 + PD-1+

−1.861

1.136

2.683

0.155

0.017–1.442

0.101

  CD3 + CD8 + PD-1+

1.004

0.775

1.679

2.729

0.598–12.464

0.195

  CD3 + PD-L1 + 

−.508

1.065

0.228

0.602

0.075–4.855

0.633

  CD3 + CD8 + PD-L1 + 

1.288

1.139

1.279

3.624

0.389–33.763

0.258

  CD3 + PD-1+PD-L1 + 

3.291

1.346

5.976

26.874

1.920–376.094

0.014

Close vs long distance from malignant cells

  CD3 + 

−0.174

1.009

0.030

0.840

0.116–6.071

0.863

  CD3 + CD8 + 

−1.376

1.253

1.206

0.253

0.022–2.945

0.272

  CD3 + CD8 + GZB + 

−2.967

1.383

4.604

.051

0.003–0.773

0.032

  CD3 + CD45RO + 

−1.248

1.281

0.949

0.287

0.023–3.537

0.330

  CD3 + CD8 + CD45RO + 

1.043

1.273

0.672

2.839

0.234–34.388

0.412

  CD3 + CD8negFOXP3 + 

2.446

1.018

5.773

11.538

1.569–84.822

0.016

  CD3 + CD45RO + FOXP3 + 

3.032

1.150

6.956

20.735

2.179–197.331

0.008

  CD3 + PD-1+

1.461

1.100

1.763

4.311

0.499–37.260

0.184

  CD3 + CD8 + PD-1+

1.754

1.012

3.007

5.778

0.796–41.956

0.083

  CD3 + PD-L1 + 

5.445

1.706

10.188

231.550

8.178–6556.148

0.001

  CD3 + CD8 + PD-L1 + 

2.316

1.920

1.455

10.134

0.235–436.512

0.228

  CD3 + PD-1+PD-L1 + 

−2.021

1.354

2.229

0.132

0.009–1.882

0.135

  1. Note: B unstandardized regression weight, SE multiple linear regression, Wald Wald test, HR hazard ratio, CI confidence interval, ADC adenocarcinoma, SCC squamous cell carcinoma, GZB granzyme B.
  2. *Boldface indicates statistically significant difference using Cox proportional-hazards model with un-adjusted P-values for clinicopathologic variables.
  3. The table shows the analysis of densities, and pattern of cellular distribution and distances relative to malignant cells to the 12 relevant cell phenotypes of the multiplex immunofluorescence panels adjusted by clinicopathologic features.
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