Fig. 4: Reusability of EAG.

a Conversions and enantiomeric excess (e.e.) values measured during 12 reaction cycles using EAG as the catalyst. n = 3. Data are expressed as the mean ± SEM. b Second derivatives of the FTIR spectra of EAG obtained after different numbers of cycles. c TEM images of the lyophilized EAG and d Confocal laser scanning microscope (CLSM) images of EAG in solution obtained (c1, d1) before use and (c2, d2) after 12 cycles. Scale bars: c 200 nm and d 4 μm. Error bars represent the standard deviations from three independent experiments. Source data are provided as a Source data file.