Fig. 1: Identification of the transcription factor MgrA as a target of S-nitrosylation. | Nature Communications

Fig. 1: Identification of the transcription factor MgrA as a target of S-nitrosylation.

From: Transcription tuned by S-nitrosylation underlies a mechanism for Staphylococcus aureus to circumvent vancomycin killing

Fig. 1: Identification of the transcription factor MgrA as a target of S-nitrosylation.The alternative text for this image may have been generated using AI.

a Summary of MS identified NO-modified proteins in S. aureus categorized by biological pathway. b Transcriptional regulators identified to be S-nitrosylated are listed. c Higher-energy collision dissociation mass spectrum of the cystine 12-containing peptide from MgrA. S-nitrosylated cysteine was labeled with isobaric iodoTMT and marked with io. The cysteine residue Cys12 of MgrA is located within the MarR-type HTH domain (d) and located at the interface of MgrA dimer (e). f S-nitrosylation of MgrA purified from the XN108 WT or Δnos strain was detected by western blot. S-nitrosylated MgrA (MgrA-SNO) was detected with an anti-TMT antibody. Total MgrA (MgrA-His) was detected with an anti-His antibody. Data are representative of n = 3 biological replicates. Source data are provided as a Source Data file.

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