Fig. 3: NO is required for S-nitrosylation on MgrA to facilitate vancomycin resistance. | Nature Communications

Fig. 3: NO is required for S-nitrosylation on MgrA to facilitate vancomycin resistance.

From: Transcription tuned by S-nitrosylation underlies a mechanism for Staphylococcus aureus to circumvent vancomycin killing

Fig. 3: NO is required for S-nitrosylation on MgrA to facilitate vancomycin resistance.The alternative text for this image may have been generated using AI.

The WT and Δnos strains (a–c) or the WT and mgrAC12S strains (d–f) were grown in MH II medium containing 2 μg/mL vancomycin in 96-well plates with shaking at 200 rpm for 48 h at 37 °C. OD600 was monitored to evaluate the growth when different concentrations of SNP were added. N-0, N-1, N-5 or N-20 represents 0, 1, 5 or 20 μM SNP respectively. Data are means of n = 3 biological replicates with SD. Two-sided two-way ANOVA, *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001; ns not significant. Source data are provided as a Source Data file.

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