Fig. 5: Removal of MgrA S-nitrosylation impairs its DNA binding activity. | Nature Communications

Fig. 5: Removal of MgrA S-nitrosylation impairs its DNA binding activity.

From: Transcription tuned by S-nitrosylation underlies a mechanism for Staphylococcus aureus to circumvent vancomycin killing

Fig. 5: Removal of MgrA S-nitrosylation impairs its DNA binding activity.The alternative text for this image may have been generated using AI.

ChIP-qPCR was used to evaluate the occupancy of endogenous WT MgrA or MgrAC12S at the promoters of lytN and sarV (a, b), and the occupancy of endogenous WT MgrA in the WT or Δnos strains (c, d). Data are means of n = 3 biological replicates with SD. Two-sided unpaired Student’s t-test, ****p ≤ 0.0001. DNA binding efficacy of WT and C12S mutant MgrA at lytN (e) or sarV (f) promoters was compared by using gel-shift assay. Different concentrations of purified MgrA or MgrAC12S protein were incubated with a FAM-labeled probe containing the MgrA binding sequence on the lytN or sarV promoter region. The probe concentrations were indicated as plytN or psarV. The upper bands represent probes bound with protein; the lower bands represent free probes. Data are representative of n = 3 biological replicates. Source data are provided as a Source Data file.

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