Fig. 4: Adult intestinal MSC derive from Gli1+ embryonic precursors. | Nature Communications

Fig. 4: Adult intestinal MSC derive from Gli1+ embryonic precursors.

From: The small and large intestine contain related mesenchymal subsets that derive from embryonic Gli1+ precursors

Fig. 4: Adult intestinal MSC derive from Gli1+ embryonic precursors.

a Representative flow cytometric analysis and b proportions of indicated cells expressing EGFP in the small intestine and colon of embryonic E12.5 Gli1-EGFP mice. Results are from 8 individual embryos, with each circle representing an individual embryo. c Workflow of lineage-tracing experiments. R26R.EYFP females were mated overnight with Gli1.CreERT2+/− males and pregnant dams injected i.p. with 4-hydroxytamoxifen (4-OHT) at E11.5. d Proportions of indicated MSC subset expressing YFP in small intestine and colon of 5.5–7-week-old Gli1.CreERT2+/−.R26R.EYFP (Cre+) and R26R.EYFP (Cre−) littermates. Results are pooled from 4 independent experiments with 10 Cre+ mice and 11 Cre− mice. Each circle represents an individual mouse. Bars represent the means and SD. See also Supplementary Fig. 4. Source data are provided as a Source Data file.

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