Fig. 4: Wetting and membrane dynamics.
From: Wetting and complex remodeling of membranes by biomolecular condensates
a After the glycinin condensate (cyan)gets in contact with the membrane (magenta) at t = 0 min, the contact angles change slowly until reaching the final morphology, which remains stable for hours; confocal (top) and bright-field (bottom) images of the same vesicle-condensate pair. b Example of the measured geometric factor \(\Phi\) for the membrane-condensate system shown in (a); DOPC membrane, 100 mM NaCl. The geometric factor was calculated using n = 3 images for each time, individual points are plotted (gray circles), the mean is indicated as a pink line and the SD is shown as a pink shadowed area. c Fluorescence recovery after photo-bleaching (FRAP) experiments show decreased fluidity in the membrane segment that is wetted by the condensate (ic segment in Fig. 2b) compared to the bare one (condensate-free, ie) on the same vesicle. For these experiments, only the membrane was fluorescently labeled to avoid interferences from condensate fluorescence. The dotted circles shown in cyan/gray indicate the bleached regions in the ic/ie membrane segments respectively. d FRAP intensity curves yield halftimes of recovery τ1/2 which show that condensate wetting slows lipid diffusion by a factor of about 2 (n = 5); see “Methods”. Scale bars: 10 μm. Data for panels b and d are provided as a Source Data file.
