Fig. 7: Dynamics of membrane ruffling driven by glycinin condensates.

a Mutual membrane-condensate molding and generation of curved structures at the membrane-droplet interface during wetting proceeds slowly (see also Supplementary Movie 3). b Zoom of the region indicated with a dotted line in (a). c Images in bright field and confocal cross section showing the two membrane segments examined by FRAP, the bare one (\({ie}\) segment in Fig. 2b) and the wetted, reticulated one (\({ic}\)). Only the membrane was labeled and it was bleached in the indicated regions. d Recovery curves for the reticulated region (cyan) and the bare condensate-free membrane (gray). From the fitting, the halftimes of recovery \({\tau }_{1/2}\) are obtained (n = 5, see “Methods”). Lipid diffusion in the membrane in contact with the condensate is twice as slow than in the condensate-free segment similarly to measurements on smooth interfaces where the slowdown is the same (compare to Fig. 4c, d). These results indicate that membrane ruffling by condensates does not alter lipid diffusion compared to that in wetted membranes where ruffling is absent. Scale bars in (a) and (c): 5 µm, in (b): 1 µm. Data for (d) is provided as a Source Data file.