Fig. 5: Substrate entry and reaction mechanism. | Nature Communications

Fig. 5: Substrate entry and reaction mechanism.

From: Structure of a eukaryotic cholinephosphotransferase-1 reveals mechanisms of substrate recognition and catalysis

Fig. 5

a–b Proposed entry pathways for both substrates. CDP-choline and DAG are shown as stick and spheres, respectively. xlCHPT1 is shown as cartoon. Proposed active site residues H133 and E129 are shown as spheres. c Proposed catalytic mechanism of CHPT1. E129 and H133 activate the 3-hydroxyl on DAG to enhance nucleophilic attack on the phosphate group of CDP-choline.

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