Fig. 3: Morphological aberrations of CIII-deficient hepatocyte nuclei. | Nature Communications

Fig. 3: Morphological aberrations of CIII-deficient hepatocyte nuclei.

From: Mitochondrial complex III deficiency drives c-MYC overexpression and illicit cell cycle entry leading to senescence and segmental progeria

Fig. 3: Morphological aberrations of CIII-deficient hepatocyte nuclei.The alternative text for this image may have been generated using AI.

a H&E-stained liver sections showing typical WT hepatocyte nuclei and the spectrum of aberrant Bcs1lp.S78G hepatocyte nuclei (anisokaryosis, karyomegaly, uneven nuclear envelope, atypical shapes, nuclear envelope blebs and invaginations, intranuclear cytoplasmic inclusion-like structures). b High-magnification images of Hoechst-stained hepatocyte nuclei. c Representative electron micrographs of hepatocyte nuclei. Two samples from the mt-Cyb p.D254N background are indicated in parentheses. Supplementary Fig. 2d shows the corresponding whole-cell cross sections. d Quantification of karyomegalic hepatocyte nuclei defined as cross-sectional area greater than 4 SDs of normal (WT hepatocytes as reference). e Quantification of misshapen hepatocyte nuclei based on nuclear envelope blebs, invaginations, and inclusion bodies from H&E-stained sections. f, g qPCR analysis of Lmna splice variants. Statistics: one-way ANOVA followed by the selected pairwise comparisons (Welch’s t-statistics). The error bars represent 95% CI of mean. All data points derive from independent mice. Source data are provided as a Source Data file.

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