Fig. 7: AOX supresses mitochondrial integrated stress response (mt-ISR) and c-MYC induction. | Nature Communications

Fig. 7: AOX supresses mitochondrial integrated stress response (mt-ISR) and c-MYC induction.

From: Mitochondrial complex III deficiency drives c-MYC overexpression and illicit cell cycle entry leading to senescence and segmental progeria

Fig. 7: AOX supresses mitochondrial integrated stress response (mt-ISR) and c-MYC induction.The alternative text for this image may have been generated using AI.

a Gene expression of mt-ISR markers in P30 liver. b, Gdf15 mRNA expression in P30 liver. c, d Western blot quantification of eIF2-α phosphorylation and ATF4 expression as readouts of mt-ISR activation. ATF4 was detected from liver nuclear fractions. e Western blot assessment of mTORC1 activation based on RPS6 phosphorylation status. f Myc mRNA expression (qPCR) in P30 liver from mice of mt-Cyb p.D254N background. g c-MYC protein expression in P30 liver from mice of the indicated genotypes. h Myc mRNA in P30 liver (qPCR), and P150 liver, kidney and heart (RNAseq) from mice with WT mtDNA. i Myc mRNA expression in P30 skeletal muscle (quadriceps). j c-MYC protein expression in P30 kidney lysates. Statistics: one-way ANOVA followed by the selected pairwise comparisons (Welch’s t-statistics). The error bars in b–j represent 95%-CI of mean. All data points derive from independent mice. Source data are provided as a Source Data file.

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