Fig. 7: Two amino acids of Ankyrin Repeat 1 determine the reduced thermal threshold of urodelan TRPV1s. | Nature Communications

Fig. 7: Two amino acids of Ankyrin Repeat 1 determine the reduced thermal threshold of urodelan TRPV1s.

From: Two single-point mutations in Ankyrin Repeat one drastically change the threshold temperature of TRPV1

Fig. 7

a IR Newt TRPV1 or its mutant (Q127R/V153K) was expressed in Xenopus oocytes and the representative heat-induced currents standardized by the capsaicin-induced currents at 50 μM were indicated. Average currents for heat stimulation at 40 °C standardized by capsaicin-induced currents were compared (n = 5). Each data point represents the mean ± SE. The statistical significance for the difference was determined by a Student’s two-sided unpaired t-test and P values were indicated in italics. Source data are provided as a Source Data file. b Y Sal TRPV1 or its mutant (Q126R/L152K) was expressed in oocytes and representative heat-induced currents are indicated. Average heat-induced currents at 40 °C were compared (n = 5). Each data point represents the mean ± SE. The statistical significance for the difference was determined by a Student’s two-sided unpaired t-test and P values were indicated in italics. Source data are provided as a Source Data file. c JC Sal TRPV1 or its mutant (Q129R/L155K) was expressed in oocytes and the representative heat-induced currents are indicated. Average heat-induced currents at 40 °C were compared (n = 5). Each data point represents the mean ± SE. The statistical significance for the difference was determined by a Student’s two-sided unpaired t-test and P values were indicated in italics. Source data are provided as a Source Data file.

Back to article page