Fig. 5: The Pi route can be integrated into the FORCE pathway to produce C2 compounds from formate. | Nature Communications

Fig. 5: The Pi route can be integrated into the FORCE pathway to produce C2 compounds from formate.

From: Engineering a new-to-nature cascade for phosphate-dependent formate to formaldehyde conversion in vitro and in vivo

Fig. 5

a Scheme of the formate activation/ reduction reactions required to produce formaldehyde and formyl-CoA to feed the downstream reactions of the FORCE pathway. b Glycolyl-CoA production by the Pi and CoA modules in vitro. Shown are single measurements, mean and standard error of technical replicates (n = 3). c Glycolate production by the Pi and CoA modules in resting cells after 3 h. Shown are single measurements, mean and standard error of biological replicates (n = 4). Negative controls as well as glycolate production at 3 and 24 h are shown in Supplementary Fig. 11. BsACS Bacillus subtilis acetyl-CoA synthetase, EcAckA Escherichia coli acetate kinase, DaArgC Denitrovibrio acetiphilus N-acetyl-γ-glutamyl phosphate dehydrogenase, LmACR Listeria monocytogenes acyl-CoA reductase, GCS glycolyl-CoA synthase, EcAldA E. coli glycolaldehyde dehydrogenase. Source data are provided as a Source Data file and in Edmond [https://doi.org/10.17617/3.BKLI0C].

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