Table 1 Kinetic parameters of acetate kinases on formate

From: Engineering a new-to-nature cascade for phosphate-dependent formate to formaldehyde conversion in vitro and in vivo

 

Km, app (mM)

kcat, app (s−1)

kcat/Km (M−1s−1)

EcAckA

106 ± 10

22 ± 0.9

2.1 × 102

StAckA

117 ± 9

16 ± 0.5

1.4 × 102

  1. Shown are the results of a Michaelis-Menten fit of technical triplicates (n = 3) with standard deviation. Curves are provided in Supplementary Fig. 1.
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