Fig. 1: Benzyl-isothiocyanate (BITC) inhibited light- and fusicoccin (FC)-induced stomatal opening in C. benghalensis.

a Schematic representation of the screening for compounds that inhibit light-induced stomatal opening. Excised C. benghalensis leaf discs were immersed in wells of a multi-well plate containing basal buffer with the test compounds and incubated for 3 h under direct light. To maximize throughput, we qualitatively evaluated the bioactivity of candidate compounds in the first and second screenings. During the third screening, the stomatal aperture was measured to determine the bioactivity of the compounds. b BITC inhibits stomatal opening in C. benghalensis in a dose-dependent manner (n = 6; 20–25 stomata per leaf disc). The values are related to the mock (DMSO) treatment. c The effect of 50 µM BITC on C. benghalensis stomatal opening induced by blue light or FC. Values are presented as mean ± SD (n = 3; 20–25 stomata per four leaf discs per replicate). d The effect of 50 µM BITC on A. thaliana stomatal opening induced by blue light or FC. Values are presented as means ± SD (n = 3; 22–44 stomata per three leaf discs per replicate). Light treatment, 150 µmol m^–2 s^–1 red light and 50 µmol m^–2 s^–1 blue light; ABA, 20 µM ABA; FC, 10 µM FC.