Fig. 1: Overview of the ADS construct and the high-throughput screening process used to convert known aptamers to molecular switches. | Nature Communications

Fig. 1: Overview of the ADS construct and the high-throughput screening process used to convert known aptamers to molecular switches.

From: A massively parallel screening platform for converting aptamers into molecular switches

Fig. 1: Overview of the ADS construct and the high-throughput screening process used to convert known aptamers to molecular switches.The alternative text for this image may have been generated using AI.

a Design of the fluorophore-labeled switching strand and quencher-labeled aptamer strand. b Target-induced conformational changes in the ADS construct result in a change in distance between the fluorophore and quencher, providing an optical readout. c Overview of the screening process. First, the switching strand library is sequenced on the flow-cell. Second, the ADS constructs are assembled on the surface of the flow-cell via addition of aptamer strands. Lastly, target-responsive molecular switches are identified by sequentially imaging the flow-cell in buffer alone and with the target molecule. Imaging data from each ADS construct cluster reveals the presence of switches for which target binding results in increased (signal-on) or decreased (signal-off) fluorescence.

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