Fig. 3: Identification and characterization of ATP aptamer switches.

a Results from the high-throughput screen of switching domains for the ATP aptamer. Orange and blue bars respectively represent the cluster intensity in buffer and 500 μM ATP. Data are presented as mean values (n = 5 replicates) and error bars represent the standard deviation of the measurements. Individual data points are overlayed as black dots. Source data are provided as a Source Data file. b Extracted images of individual ATP-responsive ADS clusters (red circle) on the MiSeq flow-cell from multiple buffer and ATP cycles. c Validation of selected ADS constructs identified from the high-throughput screen. 50 nM fluorophore - and quencher-labeled ADS construct was incubated with various concentrations of ATP and measured on a plate reader (n = 4 replicates). A two independent binding site model was used to fit the raw data and normalize the binding signals between 0 and 1, as represented by the solid lines. Data are presented as mean values and error bars represent the standard deviation of the measurements. Source data are provided as a Source Data file.