Fig. 5: Identification and characterization of phenylboronic acid-modified aptamer switches for glucose.

a Analysis of the top four aptamer switch clusters identified in our flow-cell screen. Orange and blue bars represent cluster intensity in buffer and 10 mM glucose, respectively. Data are presented as mean values (n = 4 replicates) and error bars represent the standard deviation of the measurements. Individual data points are overlayed as black dots. Source data are provided as a Source Data file. b Extracted images of clusters glu-1, −2, and −3 (red circles) on the MiSeq flow cell for both the buffer and glucose cycles. c Validation of the glucose affinity of the four aptamers shown in panel A. 50 nM labeled ADS construct was incubated with various concentrations of glucose and the fluorescence signal was measured on a plate reader. The solid lines represent the fitted single binding site model. Data are presented as mean values (n = 3 replicates) and error bars represent the standard deviation of the measurements. Source data are provided as a Source Data file.