Fig. 2: Species-specific histone modifications observed in rAAV genomes delivered with the AAV-LK03 capsid into mouse vs human cells. | Nature Communications

Fig. 2: Species-specific histone modifications observed in rAAV genomes delivered with the AAV-LK03 capsid into mouse vs human cells.

From: The AAV capsid can influence the epigenetic marking of rAAV delivered episomal genomes in a species dependent manner

Fig. 2

a H3K4me3 normalized (CPM) signal on AAV genome obtained from the Cut&Tag assay and next generation sequencing for Huh7 human and Hepa 1–6 mouse cell lines transduced with AAV-LK03 and AAV-DJ, as indicated. b Cut&Tag boxplots of normalized coverage on the AAV genome, for histone epigenetic modifications - H3K4me3 and H3K27ac (active transcription), H3K9me3 and H3K27me3 (inactive transcription) for Huh7 human and Hepa 1–6 mouse cell lines transduced with AAV-LK03 and AAV-DJ. Statistics were performed with 2 way ANOVA and only statistically significant differences are indicated. Statistic p-value * <0.05, ** <0.01, *** <0.001. H = human, M = mouse. N > = 3 biologically independent samples. Boxplots display the median (thick bar), two hinges (lower and upper hinges correspond to the first and third quartiles) and two whiskers. The upper whisker extends from the hinge to the largest value no further than 1.5 *IQR (inter-quantile range) from the hinge. The lower whisker extends from the hinge to the smallest value at most 1.5 * IQR of the hinge. Raw data for the graphs are provided in Source Data File 1. Detailed statistics for each graph are provided in Source Data File 2.

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