Fig. 4: The I3 to I2 and DH270.1 path split. | Nature Communications

Fig. 4: The I3 to I2 and DH270.1 path split.

From: Structural basis for breadth development in the HIV-1 V3-glycan targeting DH270 antibody clonal lineage

Fig. 4

A DH270 clonal tree highlighting the I3 and I2 intermediate antibodies and the mature DH270.1 (M1) antibody. B Top views of the I2 and DH270.1 contact sites. Alpha-carbons (Cα) of heavy and light chain mutations are presented as spheres. C Alignment (gp120 only) of I3 and I2 bound state structures highlighting the shift in gp120 V1 loop arrangement and contact between the I2 R84 side chain and the N137-glycan. D Alignment (gp120 only) of UCA and I2 bound structures highlighting conformational similarity between the gp120 V1 loops. E (right) Antibody Fab VH/VL centroid distance from the gp120 epitope centroid. (left) Theta vs. phi plot for the I3, I2, and DH270.1 structures. The colors of the data points match the respective heavy chain colors in the structures. F Side view comparison of the LCDR3 region epitope contact between I3 and I2 highlighting the shift in LCDR3 conformation (magenta arrow). G Top view comparison of the LCDR3 region epitope contact between I3 and I2 highlighting the shift in the aromatic residue 93 rotamer (magenta arrow). H Alignment (gp120 only) of I3 and DH270.1 bound state structures with DH270.1 map highlighting the Y106S mutation.

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