Fig. 5: The I4 to mature DH270.2 and DH270.3 transition. | Nature Communications

Fig. 5: The I4 to mature DH270.2 and DH270.3 transition.

From: Structural basis for breadth development in the HIV-1 V3-glycan targeting DH270 antibody clonal lineage

Fig. 5

A DH270 clonal tree highlighting the I4 intermediate antibody and the mature DH270.2 (M2) and DH270.3 (M3) antibodies. B Top views of the DH270.2 and DH270.3 contact sites. Alpha-carbons (Cα) of heavy and light chain mutations are presented as spheres. C (left) Alignment (gp120 only) of I4 and DH270.2 bound state structures highlighting the shift in antibody disposition (right) Alignment (gp120 only) of I4 and DH270.3 bound state structures highlighting the shift in antibody disposition. D Theta vs. phi plot for the I4, DH270.2, and DH270.3 structures. The colors of the data points match the respective heavy chain colors in the structures. E Structure overlay of the I4 and DH270.3 N301-glycans showing their change in disposition and the location of the S27 or Y27 residues on the antibody light chain. F Alignment (gp120 only) of UCA+G57R and DH270.3 bound state structures indicating similar bound state arrangements. G Comparison of the LCDR3 contact site with the gp120 protein and N301-glycan between the I4 (upper) and DH270.3 (lower) structures. H Comparison of the N332-glycan D1 arm antibody contacts between the I4 (upper) and DH270.2 (lower) structures.

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