Fig. 4: Proinflammatory and profibrotic profile of alveolar and interstitial macrophages after fibrogenic doses of IR.
From: An interactive murine single-cell atlas of the lung responses to radiation injury
a UMAP visualization of 11,678 cells from the different IM and AM subpopulations annotated by cell type. b UMAP visualization of NI (n = 5), IR5M10Gy (n = 1) and IR5M17Gy (n = 2) IM and AM annotated by time point. c Dynamics in the proportion of the IM and AM subpopulations at the different time points after IR10Gy and IR17Gy. d Automatic C3ar1 (orange) and Chil3 (orange) mRNA detection with Big-FISH in NI, IR5M10Gy and IR5M17Gy lung tissue sections. Scale bars, 10 µm. e Quantification of the C3ar1+ and Chil3+ cells in the NI, IR5M10Gy, and IR5M17Gy lung tissue sections. To compare two groups, the P value was computed with the Mann–Whitney–Wilcoxon test (two-sided test) from scipy (n/s, adjusted p value >0.05; *, adjusted p value <0.05; **, adjusted p value <0.01; ***, adjusted p value <0.001; ****, adjusted p value <0.0001). Each dot represents one analyzed image. Each color per time point represents a different biological replicate (NI n = 3; IR5M10Gy n = 3; IR5M17Gy n = 5). f Violin plot showing the single cell score calculated based on the M1 signature in the different IM subpopulations. g Violin plot showing the single cell score calculated based on the M2 signature in the different AM subpopulations. h Violin plots of M1 genes expression in the different IM subpopulations. i Violin plots of M2 genes expression in the different AM subpopulations.
