Fig. 1: Schematic representation of a Connectase-mediated ligation of two substrates, N and C (a), and AlphaFold model (b) of M. mazei Connectase (white) in complex with its recognition sequence (red, cyan).
From: Specific, sensitive and quantitative protein detection by in-gel fluorescence
Connectase forms an amide bond between its active site residue (Thr-1) and the N-terminal part of a substrate (N-Cnt), while the C-terminal part of the substrate is cleaved. The reaction is reversible so that the amide bond in the original substrate (red) can be reconstituted. Alternatively, a new fusion product with an alternative C-terminal fragment (blue) can be formed.
