Fig. 2: Schematic representation of the in-gel fluorescence detection method.
From: Specific, sensitive and quantitative protein detection by in-gel fluorescence
Connectase is pre-loaded with a fluorescent peptide substrate (1) and mixed with a sample containing a CnTagged Protein of Interest (2). After a short incubation step, the fluorescent protein of interest can be visualized on a polyacrylamide gel (3).
