Fig. 1: The expression of POLD1 was correlated with the malignant degree and prognosis of BLCA.
From: DNA polymerase POLD1 promotes proliferation and metastasis of bladder cancer by stabilizing MYC
The mRNA level of POLD1 in BLCA (MIBC or NMIBC) and normal tissues in a TCGA-BLCA (RNA-seq data) and b Zhongnan Hospital cohort (RT-qPCR data). Statistical significance was determined by two-tailed Wilcoxon rank-sum test (a) and paired two-tailed Student’s T-test (b). The mRNA level of POLD1 in different c stages (Ta, T1, T2, T3, and T4) and d pathology grades: Papillary Urothelial Neoplasms of Low Malignant Potential (PUNLMP), low grade and high grade in the UROMOL39 cohort. Statistical significance was determined by two-tailed Wilcoxon rank-sum test and Kruskal–Wallis test (c, d). The prognostic curve (disease-free survival) of different POLD1 mRNA levels in the two subtypes of e NMIBC and f MIBC in the GSE3289440 cohort was analyzed. The patients were divided into a high POLD1 mRNA level group and a low POLD1 mRNA level group according to the median POLD1 expression. The prognostic curve (overall survival) of different POLD1 protein levels in the two subtypes of g NMIBC and h MIBC in the HBlaU079Su01 cohort was analyzed. The patients were divided into high and low POLD1 protein level groups according to the median POLD1 protein level. Statistical significance was determined by the log-rank test of Kaplan–Meier analysis (e–h). The purple line represents the group with high POLD1 mRNA or protein expression and the black line represents the group with low POLD1 mRNA or protein expression. Representative pictures (left panel) and statistical figures (right panel) of immunohistochemistry staining analysis of POLD1 protein levels in different i stages (The seventh edition of AJCC: stage Ois, stage I, stage II, stage III, and stage IV), and j T stages (Tis, T1, T2, T3, and T4) in the HBlaU079Su01 cohort. POLD1 nuclear staining score = The intensity of nuclear staining × Staining positive area. Data are mean ± SD. Statistical significance was determined by the two-tailed Student’s T-test (i, j). The n number represents n biologically independent patient samples in each group. Exact n values are marked in the images. NMIBC non-muscle-invasive bladder cancer, MIBC muscle-invasive bladder cancer, CI confidence interval, inf infinite, TPM transcripts per kilobase million, FPKM fragments per kilobase million. Source data are provided as a Source Data file; ns: not significant.
