Fig. 7: The cheating behavior of DNA nano-dice system.
From: Throwing and manipulating and cheating with a DNA nano-dice
A Schematic illustration of three cheating methods for nano-dice. PT modification: phosphorothioate modification. B Schematic illustration of adsorption and desorption of original t-DNA-1/2 by adding poly A12/T12/C12/G12. C Desorption ratio of FAM labeled original DNA (group 1) from GO by poly A12/T12/C12/G12 after 60 min reaction, n = 3 independent experiments. Data are presented as mean values ± s.d. D Schematic illustration of the differences of the binding affinity between polyN12 (N = A, T, C or G) and GO. E Normalized fluorescence intensity ratio of cheating dice (A/T/C/G), n = 3 independent experiments. Data are presented as mean values ± s.d. F Schematic illustration of the differences between normal t-DNA and t-DNA with abasic sites on GO. G Normalized fluorescence intensity ratio of cheating dice (empty), n = 3 independent experiments. Data are presented as mean values ± s.d. H Schematic illustration of the differences between PO DNA and PT DNA on GO. PO DNA: DNA with phosphodiester backbone; PT DNA: DNA with phosphorothioate modification. I Normalized fluorescence intensity ratio of cheating dice (*), n = 3 independent experiments. Data are presented as mean values ± s.d. J Correlation analysis between DNA adsorption affinity and relative probability of “6”, n = 3 independent experiments. X-axis: average of the two bars in t-DNAs (F1, F2) for each cheating methods, indicative of DNA adsorption strength. Y-axis: average of the two bars in nano-dice (F3, F4) for each cheating methods, reflecting the relative probability of throwing “6” (pip). The cheating effect of each cheating dice compared with the normal dice was verified by one-way analysis of variance. *p value < 0.05, **p value < 0.01, ***p value < 0.001. Data are presented as mean values ± s.d. K The absolute probability of “1” to “6” (pips) with different cheating dice. Formula: Ppip6 = 1/6 × EF3,4 and Ppip1/2/3/4/5 = (1- Ppip6)/5 (the remaining 5 pips are of random equal probability). L Schematic illustration of cheating dice for throwing “6” (pip). Source data are provided as a Source Data file.
